Fluorescence Microscopy

Leica TCS SP8 Confocal & STED Super Resolution Microscope

The Leica TCS SP8 STED super-resolution microscope is based on the STED (STimulated Emission Depletion) principle developed by Nobel Prize laureate Stefan Hell.  The base of this system is a fluorescence confocal microscope which is suitable for use in top level biological and biomedical research and surface analysis in material science applications.

• The light sources include a pulsed 405 nm diode laser, a white light Laser which can provide excitation wavelengths from 470 nm - 670 nm, and a metal halide lamp for wide field imaging
• 592 nm and 660 nm STED depletion lasers for super resolution imaging.
• Fluorescent Lifetime Microscopy using HyD single molecule detectors
• FLIM, FCS, FLCS, FRAP and FRET measurements.
• 2 Internal standard PMT confocal detectors, 2 Internal HyD GaAsP SMD detectors for gated imaging and 1 CCD camera
• 10x air, 20x, 40x and 63x (with motorized correction collar) water, 40x, 63x and 100x oil objectives.
• Resonant scanner for imaging at 24 frames per second.
• Galvoflow, SuperZ galvanometer
• 37°C degree stage top CO² incubator for live cell imaging
• Has both high resolution and flexibility to cover a wide range of experimental setups in fluorescent microscopy

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Nikon Eclipse Ti-E Fluorescence Microscope

The Nikon TiE is fully automated inverted widefield fluorescence microscope with high-speed motorization. The Eclipse Ti-E inverted microscope offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and with its environmental control, it is perfect for live cell imaging. It has a motorised and automated XYZ, and a 37°C degree stage top CO² incubator for live cell imaging

Discovery Echo Revolution Widefield Microscope

The Discover Echo Revolution Microscope is a widefield fluorescence microscope that can operate in both an upright and inverted configuration, meaning a wide variety of sample types can be examined. 

Sources: 

• Brightfield LED Illumination 
• LED Fluorescence Module

Fliter cubes: 

• DAPI
• FITC
• TRITC
• TxRed
• CY5

Multi-Dimensional Imaging:

• Time-Lapse
• Multi-Point
• Mosaic
• Multi-Channel 
• Z-Stack
   

Cameras: 

• Cameras: Brightfield: 5MP CMOS Color 
• Fluorescence: 5MP sCMOS Mono 

Polarised and Phase contrast imaging